Encoded polymer microparticles

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Provided are: an encoded polymer microparticles comprising an encoded polymer microparticle core and a silica shell encompassing the microparticle core; and a multiplexed bioanalysis service method using the same. In addition, provided is a method for preparing an encoded polymer microparticles, comprising the steps of: providing a mixture of a radiation curable material, and a linker comprising a functional group capable of being polymerized with the radiation curable material, and an alkoxysilyl group; obtaining an encoded polymer microparticle core by applying a patterned energy to cure the mixture and encoding the same; and forming a silica shell on the encoded polymer microparticle core by treating the encoded polymer microparticle core with a silica precursor.

Coded polymer microparticles

It relates to a technique for coded polymer microparticles disclosed herein, and more particularly, excellent chemical and physical stability, mass production is possible coded polymer microparticles, to a multi-bio-assay using a preparation method thereof, and it.

Recently, the coded polymeric microparticles are prepared it is simple and easy to have a characteristic loading the code many bio-analytes (proteins, DNA, etc.) is widely used for detection. However, the polymer material itself is physically and chemically durable and can easily come off, and this strain has the disadvantage absorb analytes causing the analysis error. In addition, the combination of these is subject to limitations on the bio-materials to effect various chemical bonding methods, so is limited to a few special chemical bond method. Thus, the coding is possible, and excellent chemical and physical stability it is necessary to develop a fine grain introduction and mass production is possible in a variety of functional groups.

According to one embodiment, the coded core polymer microparticles; And there is provided a coded polymer microparticles comprising a silica shell surrounding the core fine particles.

According to another embodiment, the photo-curable material, and providing a linker comprising a horn hammul with the light-curable material and the polymerizable functional group and an alkoxysilyl group; Curing the mixture by applying a patterned energy, to obtain a polymer fine particle core coded by coding; And the encoding method of encoding a high molecular polymer microparticles by processing the coded polymeric silica precursor in the core fine particles forming a silica shell on the core fine particles is provided.

According to another embodiment of the invention, the coded microparticle core polymer, the fine particles of silica shell surrounding the core, and bio-analytical method using a multi-coded polymer microparticles containing the biomaterial bound to the silica shell, It is provided.

Figure 1 shows the different coded polymer microparticles to an embodiment of the present invention.

Figure 2 is a process flow diagram showing a method of manufacturing polymeric microparticles coded.

Figure 3 illustrates a process of creating a coded polymer microparticles according to one embodiment of the present invention.

Figure 4 is a scanning electron microscope (SEM) image illustrating a process of growing the silica shell on the surface of the actual particle.

5 is EPMA (electron probe micro-analyzer) is compared to the spectrum of the silica-coated fine particles and silica fine particles with uncoated.

6 is a photograph comparing the characteristics of a non-polymer fine particles (stars) and the polymer fine particles having a silica shell (circle shape) of the silica shell.

7 is fixed to the oligonucleotide on the polymer-coated silica fine particles surface shows a process for performing multi-DNA hybridization assay.

Figure 8 shows a multi-HPV genotyping using the silica-coated polymer microparticles coded.

Figure 9 shows the manufacture and handling of silica-coated coded "magnetic" particles.

Referring to the drawings will be described in more detail for various embodiments of the present invention. The following embodiments will be introduced that are provided as an example to fully convey the thought to be disclosed in the art. Therefore, the disclosed techniques may be embodied in different forms and should not be limited to the embodiments described hereinafter. And In the drawings, the width of the component, the length, thickness and the like may be exaggerated for convenience. When the component to be that "on" another component, which in the case as well as other components, "directly above", it includes the case that the other components in between.

Figure 1 shows the different coded polymer microparticles to an embodiment of the present invention. 1, FIG. 1A, the bottom view of FIG. 1B, and 1C are respectively A-A ', B-B', and C-C 'are cross-sectional views along the line. Referring to A of Figure 1, the coded microparticles polymer 100 includes a coded polymer microparticles core 110 and a silica shell (120) surrounding them. Core 110 may be encoded in a variety of ways known in the art. For example, the coded microparticles polymer core 110 may include a graphical code, fluorescence code, or a color code.