Multicenter protein compatible chromatography purification instrument

Medicilon is a leading provider of comprehensive, high quality recombinant protein and bioprocess services. We offer a variety of recombinant protein expression platforms along with a host of other protein services like chemical protein synthesis, protein refolding and structural biology services.Email:marketing@medicilon.com.cn web:www.medicilon.com

The utility model relates to experimental equipment which is used in life science, biological technology and medication, which is mainly used for the automatic affinity chromatography for purification of a great amount of proteins. The mainly technical characters of the utility model are that the experimental equipment is provided with five channels and five chromatography columns, the affinity chromatography for purification of the five channels can be simultaneously done, different sizes of the chromatography columns can be changed according to experimental requirements, thereby achieving the affinity chromatography for purification of proteins with big capacity, an industrial programmable controller system is adopted to automatically control the affinity chromatography for purification of proteins, the affinity chromatography for purification parameters of proteins comprise liquid flow which can be arranged by control panels. The experimental equipment achieves low production cost in technique, the affinity chromatography for purification of multi-channel big-capacity proteins is done on a single apparatus, and maintenance and operation are easy.

In the prior art, the protein purified by affinity chromatography control devices are mostly microcontroller development, small passage, not a large number of large capacity for protein purification; and procurement costs, high maintenance costs, high operating costs.

Utility Model Content:

The utility model is intended for the deficiencies of the prior art and to provide a large number of protein purification can be carried out in the large capacity, simple structure, low investment, low operating costs, low maintenance costs, the effect is much better channel protein affinity chromatography instrument.

The utility model is intended to achieve through the following measures: a multi-channel protein purified by affinity chromatography instrument, it has: housing, waste collection containers, collection containers of protein, protein container, TE container,TEA container, GLY container, programmable controllers and control circuits, flow injection pump, flow sensor, A nucleic acid protein detector, shut-off valve, chromatography, liquid splitter, protein collector, display,Liquid line, the special features are: TE container, TEA container, GLY container through the liquid line and corresponding liquid tap and shut-off valves in series access each column, a protein container is directly connected to a corresponding cut through the liquid line valve access each column; preceding column output termination flow injection pump, the injection pump flow via one output terminal connected to the liquid line shut-off valve followed by the waste collection container; secondly an output through the liquid pipe Road access off valve followed by protein collection container; it is again connected to one end of the liquid line shut-off valve by nucleic acid protein detector followed, and then the liquid line connected flow sensor and collector.

Compared with the prior art, the use of the utility model proposed multi-channel protein purified by affinity chromatography instrument, which has a simple structure, low cost, easy to maintain, easy to operate and so on; especially suitable for a large number of large-capacity protein purification.

With reference to the following detailed description of specific embodiments:

In the present utility model is shown in Figure 3 an embodiment of FIG. 1 ~ Fig, multi-channel protein purified by affinity chromatography apparatus comprising: a housing, waste collection container, collecting container proteins, protein container, TE container, TEA container, GLY container, programmable controller and its control circuit, flow injection pump, flow sensors, nucleic acid protein detector, shut-off valve, chromatography, liquid splitter, protein collector, display, liquid line. Column can be broadly divided into the operating area, programmable logic controller (PLC) control zone, three zone traffic detection and control zone.

Programmable Logic Controller (PLC) Q includes five expansion IO modules, two analog modules; OUT terminal 10 input module are shut-off valve and protein S), TE Input cutoff wide ⑥, GLY enter the shut-off valve 0), TEA input shut-off valve ⑧, flow injection pump O), protein output cut wide CK receives the type output shut-off valve Q, waste output shut-off valve O, the collector is connected via an intermediate relay 0; signal OUT nuclease G protein UV detector and flow sensors 0 are connected to the IN side two analog modules; programmable logic controller (PLC) Q features are process control, process monitoring, dynamic modification parameters, fault alarm and display.

TE container, TEA container, GLY container through the liquid line and corresponding liquid tap and shut-off valves in series access each column, a protein container is directly connected via a liquid line shut-off valve corresponding to access each column. The aforementioned liquid in the column was eluted and protein binding. The process uses a programmable logic controller (PLC) control, process control parameters and default values ​​can be dynamically set by the man-machine interface; flow injection pump to control the flow of different liquids; TE, TEA, GLY and protein automatically in accordance with the PLC program handover; TE during elution, TEA, GLY liquid through the column, the flow syringe pump, shut-off valve to a waste collection container directly output container; stage in the protein binding, protein through the column, the flow syringe pump, shut-off valve Direct output to the protein collection container.

When samples arrive after closing stages, nucleic acid protein UV detector detects the protein readings pipe signals to analog signals PLC module, PLC sample presets based on the received signal and began to collect the sample and stop receiving preset value comparison to determine whether to close the sample; when the samples did not meet the income, GLY after liquid chromatography, flow injection pump, UV detector nucleic acid protein flowing waste container; when it reaches close sample, after liquid chromatography, flow injection pump, shut-off valve and then the nucleic acid protein UV detector, flow sensor to the sample collector automatic admission.

Described above in connection with the accompanying drawings of the utility model to one embodiment, examples are given of the structure does not constitute a limitation of the utility model, according to the actual production needs, determine the number of the desired column,

And likewise adjusted its various types of valves and flow sensors connected and the implementation of process control by PLC. Art skilled in the art that various changes and modifications may be within the scope of the appended claims.