A method of establishing a mouse model of gastric cancer

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The present invention discloses a method for establishing mouse model of gastric cancer. Take 4-week-old C57BL / 6 mice, the mice were able to bound their activities but as for the tubular device can freely breathe, 6 to 8 hours per day for 25 to 30 days for a period of stress, stress in each cycle interval 5 to 7 days, a total of 2-3 cycles of stress; stress at the same time, mice were given drinking water containing N- nitroso compounds, administered at weekly intervals and then administered one week dosing interval and given normal drinking water, A total of 5 weeks; after the administration of conventional breeding 35 weeks, the mice get stomach cancer. Previously reported after MNU treatment alone C57BL / 6 mice formation of gastric cancer is about 30%, of the present invention will MNU treatment and stress management after the combination, the formation rate of gastric cancer C57BL / 6 mice increased to 60%.

The present invention belongs to the field experiments, set and method for establishment of a mouse model of gastric cancer.

Currently, gastric cancer model mice were divided into spontaneous tumor models and xenograft model. Xenograft model is used in the formation of tumors in immunodeficient mice (such as athymic nude mice, SCID mice), blocks tumor cells or tumor transplanted into immunodeficient mice after. Gastric spontaneous tumor mouse model is divided into genetically engineered mice model and environmental factors to stimulate gastric stomach cancer model. Now in the world there are clever genetically engineered mouse models of gastric cancer: INS-GAS mice, TFF-lknockout RunnX3knockout mice and mice. These mice were transported using mouse embryonic stem cells homologous recombination, a specific gene in the mouse genome knockout or knock. At present, the success of gastric cancer in mice induced by environmental factors include: secluded port Helicobacter (Cancer Res65: 10709-10715), nitroso compound MNNG (Gann70 :: 343-352) and MNlXjpn J Cancer Res84: 1258 - 1264). Secluded port Helicobacter in vitro amplification, fed to mice to make colonization in the stomach. MNNG and MNU is mixed in drinking water of mice, the mice induced gastric continuous drinking.

mouse xenograft model of gastric cancer has been highly controversial, because the graft itself is not formed tumors in mice, and can not reflect the real process of tumor formation in vivo. In addition, the current field of oncology research found that the body's immune system microenvironment plays an important role in tumor formation, and immunodeficient mice lack a normal immune system, therefore, the use of transport models to study bound to produce errors.

spontaneous tumor model because of its similar process occurs in vivo human tumors and is widely recognized form of gastric cancer is a process of many factors, including gene mutations, stimulating environment, etc., and genetically engineered mouse models of gastric cancer only a gene mutation is not the important environmental considerations into, so environmental factors stimulate gastric model is closer to nature than the genetically engineered mouse models of human gastric cancer formation.

Currently, only one reported the successful use of the quiet port of Helicobacter-induced stomach cancer in mice, and is mainly intraepithelial neoplasia DMNNG used mouse model induced by gastric success rate reported in the literature vary, there are two papers reported the successful induction, the rest were not successful induction. MNU is the current rate of tumor formation has proved relatively stable, the rate of tumor formation in C57BL / 6 mice approximately 30〇 about / square.

The present invention is directed to the above-mentioned disadvantages of the prior art and to provide a method of establishing a mouse model of gastric cancer.